NFAN(E)-010 Phospholipids Analysis
The cell membrane, composed of a variety of lipids, is involved with cellular signal transduction. For example, glycolipids act as receptors and ligands on the cell membrane and as signal transduction molecules, which aggregate to form a platform for signal transduction referred to as the lipid microdomain, or raft (PDF 348 KB).
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NFAN(E)-009 Impurity Analysis of Erythromycin
The NanoFrontier LD is a hybrid LC/MS/MS system consisting of a linear ion trap and a time-of-flight (TOF) mass analyzer. The linear ion trap allows MSn analysis and the TOF analyzer affords high mass-accuracy/resolution over a wide, quantitative dynamic range. This combination provides a highly reliable system for structure analysis with quantitative capabilities (PDF 375 KB).
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NFAN(E)-008 Biomarker Discovery in Serum Protein
To provide an effective tool for biomarker research in clinical proteomics, we developed a platform for discovering biomarker by combining a two dimensional liquid chromatograph (2D-nanoLC), a linear-ion-trap time-of-flight mass spectrometer (LIT-TOF), and an Information Based Acquisition (IBA) function (PDF 415 KB).
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NFAN(E)-007 Structural Assignment of Oligosaccharides
Direct Structural Assignment of N-linked and O-linked glycopeptides by MSn Mass Spectrometry. Over 50% of proteins in general, and over 90% of proteins on cell surface, exist as glycoproteins, that is, proteins post-translationally modified with glycans. Those modifying glycans are not only directly involved in the structural stability and mechanism of the proteins, but also play antenna-like roles in the cell-to-cell interaction and signal transduction (PDF 853KB).
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NFAN(E)-006 Toxicological Studies
A common conventional method for protein expression analysis in toxicological studies is to separate the expressed proteins by two-dimensional electrophoresis (2DE) and identify the differences between samples. The throughput of this technique, however, is limited by separation reproducibility and time consuming procedures (PDF 378 KB).
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NFAN(E)-005 Oligosaccharides Analysis
Mass spectrometry analyses of various N-glycans binding to proteins/peptides are highly desirable for elucidating their biological activities. An approach based on CID MSn spectra (called MSn spectral matching) acquired by ESI-IT MS and ESI-IT TOF MS in both positive and negative ion modes has been proposed as a simple structural assignment method for N-glycans with and without cleaving from the peptide (PDF 776 KB).
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NFAN(E)-004 Phosphoproteome Analysis
Protein phosphorylation plays a critical role in intracellular signal transduction; as such, it is one of the most frequently studied post translational modifications. For example, when a cell membrane receptor is stimulated, tyrosine kinase is activated, which causes the phosphorylation of proteins downstream in the signal pathway and the subsequent activation of transcription or cytoskeletal remodeling (PDF 369 KB).
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NFAN(E)-003 N-Glycans using nanoESI/Linear-IT-TOF MS and MSn
Positive- and negative-ion MSn spectra of N-linked glycopeptides binding a neutral and a sialylated glycan were acquired by using electrospray linear-ion trap time-of-flight mass spectrometry (ESI-LITTOFMS) and collision-induced dissociation (CID) with He as a collision gas (PDF 647 KB).
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NFAN(E)-002 Biomarker using nanoLC/LIT-TOFMS & IBA
Serum protein profiling using mass spectrometry is one of the most promising approaches for biomarker identification. We adopted a nanoLC/LIT-TOFMS system and newly developed software known as Information Based Acquisition (IBA) to identify biomarkers in human serum (PDF 647 KB).
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NFAN(E)-001 N-Glycans
The four isomers of 2-aminopyridine (PA)-derivatized monosialylated oligosaccharides (complex-type Nglycans) were analyzed using nanoHPLC/ESI-ITTOFMS in the negative-ion mode. It was shown that the negative-ion MS2 and MS3 spectra are reproducible; this is an essential element for MSn spectral matching (PDF 419 KB)
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NFTN(E)-002 NanoFrontier ECD
Here we describe a new compact device for electron capture dissociation (ECD) analysis of large peptides and post-translational modifications of proteins, which can be difficult to analyze via conventional dissociation techniques such as collision induced dissociation (CID) (PDF 752 KB).
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NFTN(E)-001 High-Resolution Multi-Step Tandem MS
Mass spectrometers are effective tools to identify and quantify unknown molecules, such as disease-related proteins and small molecules in pharmaceutical research and medical diagnosis. In addition, mass spectrometry can be particularly powerful when analyzing molecules with complex structures, such as post-translationally-modified proteins (PDF 344 KB).
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