Nano Flow LC System : Hitachi High Technologies America

Nano Flow LC System

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Product Number:NanoFrontier nLC

Gradient reproducibility at a flow rate of 50 nL/min (n=5)

[Conditions]

Flow rate: 50 nL/min
Solvents: A - Water; B - 80% CH3CN 50mg/mL
Gradient settings: 0 min (B=10%) ’! 40 min (B-100%); Detector UV 273 nm (31-nL cell, 1-nm slit)

An exampleof LC/MS measurement at a flow rate of 50 nL/min

[Conditions]

Sample: BSA
Sample amount: 1¼^LL
Flow rate: 50 nL/min
Mobile phase: A) 0.1% formic acid in 2% acetonitrile; B) 0.1% formic acid in 98% acetonitrile
Gradient settings: A/B=98/2 (0 min) ’! 60/40 (60 min) ’!
2/98 (60~70 min)

Retention time reproducibility for each peptide (n=6)

*(Reference)

When measuring the same sample by using the conventional LC system at 100 ¼^LL/min, the retention time was 0.1% RSD.